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[Molecular Biology]
[Recombinant DNA Techniques]
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[Research]

General protocols

bulletLab writeups [9/22/10]

DNA analysis

bulletAgarose gels-TBE [3/7/07]
bulletAgarose gels-TAE [3/7/07]
bulletDNA ladders [2/16/06]
bulletPCR (FideliTaq or AmpliTaq) [9/14/05]

RNA/DNA purification

bulletClontech minipreps [8/06/10]
bulletQiagen minipreps [3/19/04]
bulletQiagen minipreps-Thermoplasma [4/30/03]
bulletSoil DNA purification [4/30/03]
bullet Viral RNA/DNA purification [7/24/12]
bullet Centrisep purification [4/19/10]

RT-PCR

bulletRT-PCR Invitrogen [3/22/07]
bullet RT-PCR Takara BluePrint [7/24/12]

Cloning

bullet Cloning blunt ended DNA (ZeroBlunt-Kan) [7/24/12] (ZeroBlunt-Amp) [7/24/12]
bullet Cloning standard PCR fragments (TopoTA-Kan) [7/24/12]

DNA sequencing

bulletABI 377 procedures
 
bulletPCR sequencing [9/30/04]
bullet Sequencing cleanup-Centrisep [10/23/03]
bullet Sequencing-place on combs [7/9/03]
bulletSequencing (all three procedures) [9/30/04]
 
bulletBeckman procedures
 
bullet Templiphi amplification of plasmids [9/12/06]
bullet PCR sequencing-Templiphi templates [9/12/06]
bullet PCR sequencing-Miniprep templates [7/24/12]
bullet Ethanol precipitation and sequencing [7/24/12]

 

bulletGeneral procedures

Mammalian cell culture

bulletFeLV cell cultures [9/19/03]
bulletFeLV 6-well plates [11/7/03]

 Last updated on: July 24, 2012

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Copyright 2013 by Dr. Dennis Revie, all rights reserved.
For problems or questions regarding this web contact: revie@clunet.edu.